Immobilized fluorogenic substrates for enzymes; and processes for their preparation
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United States of America Patent
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Jan 30, 1990
Issued Date -
N/A
app pub date -
Jun 3, 1985
filing date -
May 31, 1985
priority date (Note) -
Expired
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Abstract
An immobilized fluorogenic substrate useful in processes for identifying and quantifying, intra- and extra-cellularly, and mammalian body fluids, as well as animal cell abstracts is disclosed. The immobilized fluorogenic substrate has the structure R.sub.1 --NH--R.sub.4 --R.sub.2 --R.sub.3 wherein R.sub.1 represents an enzyme-specific oligopeptide; R.sub.2 represents a spacer group which is methylenecarbonyloxy, a methylenecarboxamido, or a methylanesulfonamido group attached to a polymethylene chain which itself has a functional group suitable for coupling with a polymer; R.sub.3 represents a biologically inert polymer; and R.sub.4 represents a fluorogenic moiety.
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