Importance

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Abstract

For selectively and reversibly adsorbing tPA, pro-tPA or mixtures thereof, from an aqueous medium, for example, derived from a melanoma cell culture, the aqueous medium is intimately contacted with an affinity reagent comprising an immobilized Kunitz-type inhibitor, substantially as occurs in and is extractable from seeds of an Erythrina species, e.g., Erythrina latissima, having Erythrina species, the following combination of characteristics: (a) it is an inhibitor of the Kunitz-type; (b) it inhibits trypsin; (c) it inhibits plasmin; (d) it has no effect on urokinase; (e) it inhibits tPA; and (f) in its immobilized form, it is a selected adsorbent for tPA and its precursor pro-tPA, to thereby cause the tPA, pro-tPA or mixtures thereof to become selectively adsorbed on the affinity reagent.

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First Claim