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Abstract

A method for carrying out a nucleic acid hybridization test to detect a target nucleic acid sequence, the method including the steps of (1) providing a polymer molecule bonded to a first single-stranded nucleic acid sequence which is either (a) a first probe capable of hybridizing to the target nucleic acid sequence, or (b) a second nucleic acid sequence capable of hybridizing to a third nucleic acid sequence bonded to a first probe capable of hybridizing to the target nucleic acid sequence; provided that, where the polymer molecule comprises DNA, the DNA is of heterogeneous base sequence; (2) where the polymer used in step (1) is bonded to (b), providing the first probe bonded to the third nucleic acid sequence; (3) denaturing the target nucleic acid sequence; (4) contacting the denatured target nucleic acid sequence with the polymer molecule bonded to the first single-stranded nucleic acid sequence and, if the first nucleic acid sequence is (b), to the first probe bonded to the third nucleic acid sequence; and (5) detecting complexes between the target nucleic acid sequence and the polymer molecule.

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First Claim