Method and reagent for determination of an analyte via enzymatic means using a ferricyanide/ferric compound system
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United States of America Patent
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May 29, 1990
Grant Date -
N/A
app pub date -
Apr 14, 1989
filing date -
Apr 14, 1989
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A composition useful in determining analytes, such as glucose or cholesterol, is disclosed. The composition contains an enzyme which specifically reacts with the analyte to be determined, a soluble ferricyanide compound which is reducible to form a ferrocyanide, a soluble ferric compound which provides ferric ions for reacting with ferrocyanide to form a reaction product, and a buffer which does not prevent formation of this reaction product. The composition has a pH of from about 3.0 to about 6.0.
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