Process for forming liposomes
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United States of America Patent
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Jun 12, 1990
Issued Date -
N/A
app pub date -
Jul 25, 1988
filing date -
Dec 10, 1986
priority date (Note) -
Expired
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Abstract
The present invention relates to a process for forming stable, uniform unilamellar or oligolamellar liposomes which encapsulate a macromolecule. However, unlike prior processes, the present method maintains a temperature below the phase transition temperature of the lipid used to make the liposome both during the hydration of the lipid and, if used, during an extrusion step. In addition, the liposome is formed and the macromolecule encapsulated in a low ionic strength solution, then they are dialyzed against a high ionic strength solution.
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