Purification of apase-1.sub.1 and retrieval of the nematode resistance gene
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United States of America Patent
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Jun 12, 1990
Grant Date -
N/A
app pub date -
Mar 30, 1987
filing date -
Mar 30, 1987
priority date (Note) -
Expired
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Abstract
An isoenzyme from tomato, acid phosphatase-1 isoenzyme (Apase-1.sup.1), has ben purified to homogeneity and is subjected to amino acid sequencing and used to prepare anti-Apase-1 antibodies. The amino acid sequence permits design of probes to recover Apase-1.sup.1 -encoding cDNA; the antibodies are also useful for this purpose. The cDNA is useful to recover the genomic DNA encoding Apase-1.sup.1, which can then be used in walking or jumping techniques to recover the genomic DNA which confers nematode resistance, since this DNA resides immediately adjacent to the Apase-1.sup.1 gene on chromosome 6 of Lycopersicon esculentum.
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