Method of isolating and purifying nucleic acids from biological samples
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United States of America Patent
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Jun 19, 1990
Grant Date -
N/A
app pub date -
Dec 1, 1986
filing date -
Dec 1, 1986
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The method of this invention is applicable to rapid separation, isolation, and purification of DNA or RNA from biological samples. The DNA/RNA may be in double-stranded or single-stranded form. The method is particularly advantageous for resolving genetic DNA or RNA found in bacteria, virus, and mammalian cells, and for use with samples of human bodily fluids and tissues, including stool, sputum, urine, and blood samples. DNA or RNA can be separated effectively from interfering components, particularly proteins, biological pigments and mucopolysaccharides. The method of the present invention can utilize commercially available strong or weak anion exchanger materials with selected solutions of known ionic strength for adsorption and elution.
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