Method for the determination of bilirubin in solution
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United States of America Patent
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Jun 26, 1990
Grant Date -
N/A
app pub date -
Jan 28, 1988
filing date -
Jan 28, 1988
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A novel analytical method for bilirubin in biological fluids. Bilirubin is catalytically oxidized to biliverdin in the presence of bilirubin oxidase (BOX) and the latter is revertedly back-converted to initial bilirubin in the presence of suitable reducing agents. The corresponding oxidation of said reducing agents provides a signal which is monitored and provides the desired analytical data. When using selected organic fluoro-compound, said signal is provided by the release of F.sup.- ions which can be measured electrometrically.
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