Stable chromogenic substrate mixture of indoxyl phosphate and tetrazolium salt, method of making and using same in biological and diagnostic assays
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United States of America Patent
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Aug 7, 1990
Grant Date -
N/A
app pub date -
Dec 30, 1988
filing date -
Jan 3, 1988
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A stable chromogenic substrate mixture of indoxyl phosphate and tetrazolium salt is prepared by dissolving the phosphate in DMF and combining the same with Tris buffer containing magnesium chloride and followed by combination of the same with a DMF solution of tetrazolium salt. The resulting mixture is stored below room temperature in the absence of light.
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