US Patent No: 4,954,264
Number of patents in Portfolio can not be more than 2000
Apparatus for separating mononuclear cells from blood and method of manufacturing and using the same
Stats
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Sep 4, 1990
Issued date -
Feb 2, 1989
filing date -
07/305,953
serial no -
Expired
status
Importance
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Abstract
A device for separating mononuclear cells from blood includes a collection tube, a first layer of a liquid density gradient material placed in the tube, a second layer of a gel-like substance placed in the tube and situated above the first layer, a porous foam member placed in the tube and situated above and in contact with the second layer of gel-like substance, and a third layer of a Newtonian gel-like substance placed in the tube above the porous foam member. The gel-like substance of the second layer has a lower specific gravity than that of the liquid density gradient material of the first layer so that it floats on top of the first layer. The gel-like substance of the second layer becomes less viscous when heated such that it is absorbed by the porous foam member and forms a hydraulic barrier between the liquid density gradient material of the first layer and a blood sample placed in the collection tube.
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First Claim
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International Classification(s)
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Cited Art
| Patent Info | (Count) | # Cites | Year |
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| 4,190,535 Means for separating lymphocytes and monocytes from anticoagulated blood | 61 | 1978 | |
| 4,350,593 Assembly, compositions and method for separating blood | 49 | 1979 | |
| 4,417,981 Blood phase separator device | 77 | 1981 | |
| 4,640,785 Separation of lymphocytes and monocytes from blood samples | 19 | 1984 | |
| 4,816,168 Separation of lymphocytes and monocytes from blood samples | 27 | 1986 | |
| 4,844,818 Method for separating the cellular components of blood samples | 54 | 1987 | |
| 4,818,418 Blood partitioning method | 43 | 1988 | |
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| 4,435,293 Particle washing system and method of use | 25 | 1981 | |
| 4,436,631 Multiple particle washing system and method of use | 32 | 1982 | |
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| 4,457,782 Composition for partitioning blood components | 20 | 1981 | |
| 4,534,798 Composition for partitioning blood components | 13 | 1983 | |
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| 4,824,560 Separation of materials from a liquid dispersion by sedimentation | 27 | 1986 | |
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| 4,751,001 Blood partitioning apparatus | 34 | 1984 | |
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| 4,295,974 Blood sample collection and phase separation device | 26 | 1980 | |
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| 4,487,700 Method and apparatus for separating lymphocytes from anticoagulated blood | 25 | 1983 | |
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| 4,310,430 .alpha.-Olefin-dialkylmaleate-based liquid separating agent | 23 | 1980 | |
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| 4,255,256 Medium for the separation of human blood leucocytes | 28 | 1979 | |
| 4,640,783 Ozone injection method and apparatus | 17 | 1985 | |