Liquid chromatography using microporous hollow fibers
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United States of America Patent
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Sep 18, 1990
Grant Date -
N/A
app pub date -
Nov 15, 1988
filing date -
Nov 15, 1988
priority date (Note) -
Expired
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Abstract
Liquid chromatography separations of solutes are achieved using porous hollow fibers. The pores of the hollow fibers immobilize a solute-absorbing phase (preferably organic) which has a greater absorbance affinity towards at least one solute in a mixture of solutes. By passing the solute mixture through the central lumen of the hollow fibers, chromatographic separation are realized due to the greater retention time of that solute with which the immobilized phase has greater absorbance affinity. The immobilized phase may be a liquid organic which may contain a surfactant so as to form reversed micelles or it may be in the form of a polymeric gel. Separations of biological species (e.g., proteins) may thus be accomplished by means of the present invention.
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