Continuous sonication method for preparing protein encapsulated microbubbles
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United States of America Patent
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Sep 18, 1990
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app pub date -
Sep 14, 1988
filing date -
Sep 14, 1988
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Abstract
An ultrasonic imaging agent is produced by a continuous sonication processing of an aqueous solution of heat-denaturable biocompatible protein. The solution is carefully preheated to a temperature of incipient protein denaturation without forming insolubilized protein. A gaseous fluid, preferably air, is added to the solution. In the sonication, the air-containing solution is foamed, increasing the formation and concentration of microbubbles, and the solution is further heated to insolubilize a portion of the protein, thereby encapsulating the microbubbles and forming particulate microspheres.
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