Fast photochemical method of labelling nucleic acids for detection purposes in hybridization assays
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United States of America Patent
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Sep 25, 1990
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N/A
app pub date -
Oct 9, 1987
filing date -
Jul 14, 1983
priority date (Note) -
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Abstract
A labeled nucleic acid probe comprising (a) a nucleic acid component, (b) a nucleic acid-binding ligand photochemically linked to the nucleic acid component, and (c) a label chemically linked to the nucleic acid-binding ligand. The label can be a specifically bindable ligand such as a hapten or biotin, an enzyme such as a .beta.-galactosidase or horse radish peroxidase, a fluorescent radical, a phycobiliprotein, a luminescent radical, or a radioisotope. The probe can be used in assays of nucleic acids, taking advantage of the ability of the nucleic acid component to hydridize.
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