Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
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United States of America Patent
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Oct 23, 1990
Issued Date -
N/A
app pub date -
Jun 17, 1987
filing date -
Aug 22, 1986
priority date (Note) -
Expired
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Abstract
A process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers and extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence. The amplified sequence can be readily detected. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.
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