Fluorescence immunoassay using water insoluble dyes
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United States of America Patent
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Dec 18, 1990
Grant Date -
N/A
app pub date -
Oct 19, 1987
filing date -
Oct 19, 1987
priority date (Note) -
Expired
status (Latency Note)
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Abstract
In a method for fluorescence immunoassay of an analyte, an antianalyte attached to the surface of a solid support is contacted with the analyte and a tracer which includes a substantially water insoluble fluorescent dye occluded in the nonaqueous portion of a sac. After binding reactions involving the antianalyte, analyte and tracer, the solid support is separated, excitation light is applied and fluorescence from dye in intact sacs bound to the solid support is measured and compared to fluorescence measured when known quantities of analyte are assayed. The invention includes a kit of materials useful in performing an immunoassay in accordance with the method of the invention.
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