Fluorescent stokes shift probes for polynucleotide hybridization
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United States of America Patent
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Feb 26, 1991
Issued Date -
N/A
app pub date -
Apr 13, 1990
filing date -
Dec 23, 1985
priority date (Note) -
Expired
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Abstract
Fluorescent stokes shift probes for polynucleotide hybridization assays are designed to provide predetermined nucleotide base unit spacings between the donor and acceptor fluorophores. When the probes are hybridized to the target polynucleotide the fluorophores paired for non-radiative energy transfer are separated by 2 to 7 nucleotide base units. The fluorophores are attached to the DNA or RNA probes by linker arms having lengths of 4 to 30 Angstroms. Fluorescein is a preferred donor for use with a Texas Red acceptor.
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