Cloning vector, molecules of recombinant DNA, bacillus subtilis strains transformed with the said molecules and methods for the expression of heterologous genes and the production and secretion of proteins coded by the said genes
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United States of America Patent
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Jun 4, 1991
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N/A
app pub date -
Aug 17, 1989
filing date -
Jul 10, 1985
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Abstract
A description is given of a cloning vector useful for the expression and secretion of heterologous proteins in Bacillus subtilis, molecules of recombinant DNA formed by the said cloning vector, expediently cut at a restriction site located within the neutral protease gene, and by the sequence of heterologous DNA which codes for the protein of interest, Bacillus subtilis cells transformed with the said molecules and capable of expressing the sequence of heterologous DNA and of producing and secreting the protein coded by the said sequence of heterologous DNA in high yields. The proteins obtained in this manner are useful in the fields of pharmaceuticals and foods, or as compounds for the production of substances which are normally obtained by chemical synthesis.
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