Transformation, somatic embryogenesis and whole plant regeneration method for Glycine species
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United States of America Patent
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Jun 18, 1991
Issued Date -
N/A
app pub date -
Jun 15, 1987
filing date -
Aug 4, 1986
priority date (Note) -
Expired
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Abstract
A method for somatic embryogenesis of soybean, (Glycine max), Glycine soja and other Glycine species is provided using immature cotyledon tissue, preferably with the embryonic axis removed, comprising culturing said tissue on a medium containing auxin, preferably NAA at a concentration of at least about 15 mg/l. A further method for such somatic embryogenesis is provided wherein the culture medium contains a synergistically acting lowered carbohydrate and auxin concentration. Particularly embryogenic cells of such tissue are identified and improved maceration methods for contacting such cells with regeneration and transformation media are disclosed. Methods for transforming somatic tissue from soybean and other Glycine species are also provided. Whole, fertile, transformed plants are obtained.
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