Microorganism immobilized in a gel prepared from polyvinyl alcohol and a polysaccharide
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United States of America Patent
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Jul 23, 1991
Grant Date -
N/A
app pub date -
Jul 26, 1988
filing date -
Aug 10, 1987
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A hydrated gel of high water content and high porosity containing an immobilized microorganism is prepared by dropwise addition of an aqueous solution containing a microorganism, polyvinyl alcohol and a polysaccharide to an aqueous solution containing polyvalent metal ions to gel the polysaccharide and form spherical gel beads, and subjecting the beads to at least one cycle of freezing and thawing to gel the polyvinyl alcohol. The polysaccharide can be sodium alginate and the polyvalent metal ions can be provided by calcium chloride. Freezing is at a temperature not higher than -5.degree. C., and the cycle of freezing and thawing is preferably repeated at least two times.
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