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Abstract

A method for the production of antibodies to an immunogen, wherein hybridoma culture supernatants are screened using cells infected with a virus recombinant encoding one or more epitopes of the immunogen. The method disclosed is particularly advantageous as: 1) cells infected with the virus recombinant can provide a good supply of an antigen; 2) the antigen is expressed in a mammalian cell and it is therefore synthesized and processed in a form essentially identical to the antigen found in a natural infection; and 3) the infected cells may be subjected to the same pre-treatments as applied to test sample tissues before their use in the hybridoma screening procedure. This ensures that the antibodies selected will recognize epitopes resistant to these pre-treatments and which may therefore be expected to be present on the test samples as prepared for analysis.

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