Enhancement of cell growth by expression of a cloned hemoglobin gene
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United States of America Patent
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Sep 17, 1991
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app pub date -
Jan 24, 1989
filing date -
Oct 23, 1987
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Abstract
The invention relates to nucleotide sequences, including a substantially purified gene which codes for an oxygen-binding protein, and a gene promoter/regulator which is useful in subjecting the translation/transcription of DNA sequences to selective regulation by external control, and plasmid vectors containing those nucleotide sequences, which are valuable bioprocessing catalysts for enhancing the growth characteristics of cells, and increasing production of various proteins and metabolites of those cells. Methods for the use of these nucleotide sequences and related plasmids for a range of applications including oxygen supply to cells, growth enhancement, expression of various gene products, enhancement of oxygen-requiring processes, binding and separation of oxygen from liquids and gases, and a range of oxidative reactions are also disclosed.
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