Method for discriminating between intact and damaged cells in a sample
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United States of America Patent
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Oct 15, 1991
Grant Date -
N/A
app pub date -
Jun 13, 1988
filing date -
Jun 13, 1988
priority date (Note) -
Expired
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Abstract
A method is disclosed for the discrimination between intact and damaged cells in a sample, and more particularly, the method uses a vital nucleic acid dye to selectively stain intact versus damaged cells which then may be counted and sorted by flow cytometry means. The method also may be used in conjunction with fluorescently labelled monoclonal antibodies to simultaneously identify cellular antigens.
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