Method for the simultaneous determination of DNA sequence variations at a large number of sites, and a kit suitable therefor
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United States of America Patent
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Nov 26, 1991
Grant Date -
N/A
app pub date -
May 1, 1989
filing date -
May 2, 1988
priority date (Note) -
Expired
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Abstract
This invention relates to a method of simultaneously determining DNA sequence variations on a large number of loci, and a kit suitable therefor. The method comprises an electrophoretic separation of restriction fragments in two dimensions on the basis of two independent criterions, namely, length and base pair sequence, and a detection of separated fragments by means of a labelled probe comprising one or more units of minisatellite sequences. Preferred probes are GC-rich minisatellite core sequences.
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