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Abstract

A method of staining a nucleic acid sequence which is fixed on a solid support is described wherein an enzymatic system which fixes this sequence is employed to detect it by non-radioactive labelling, the enzymatic system being subsequently reacted with chromogenic substrate to produce a colored product. A developer solution is used comprising a sulfur organic compound constituting the substrate which is adapted to be hydrolized by the enzyme to form an organic thiol, and a metallic compound, for example a gold compound. The metallic compound is soluble and stable in aqueous solution and can react with the organic thiol to form a metal-organic compound complex having a characteristic color which precipitates in situ, which metal-organic complex subsequently may be decolorized with a reducing agent to thus allow reuse of the solid support. A set, or kit, of reactants for carrying out the method also is disclosed.

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First Claim