Apparatus and method for immunohistochemical staining

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United States of America Patent

PATENT NO 5073504
SERIAL NO

07459747

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Abstract

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A device and method for automating immunohistochemical staining of biological material on glass slides is presented. The slides are placed on the base with the tissue section facing upwards. A 'chamber' consisting of a rectangular plastic box with a hinged overhead door is clamped directly over the tissue section. A rubber gasket seals the chamber--slide interface. Reagents are dropped on to the tissue section by means of the hinged overhead plastic door. When the door is closed, the chamber is sufficiently sealed so as to prevent evaporation. Washing of the tissue section is accomplished by the addition and aspiraton of buffer through ports in the chamber. The ports are connected via flexible tubing to a manifold. Because the wash steps are performed in tandem and do not require manual manipulation of slides, a significant time savings is realized. A mesh may be placed between reagents and the surface of the slide to spread a reagent over the surface of the slide.

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Patent Owner(s)

Patent OwnerAddress
DAKO DENMARK A/SPRODUKTIONSVEJ 42 GLOSTRUP D-2600

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Bogen, Steven A 59 Millstone Rd., Readville, MA 02136 25 1503

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