Coumarin derivatives for use as nucleotide crosslinking reagents
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United States of America Patent
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Jan 21, 1992
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N/A
app pub date -
Apr 5, 1989
filing date -
Apr 5, 1989
priority date (Note) -
Expired
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Abstract
A photoactivatible nucleoside analogue is disclosed, comprising a coumarin moiety linked through its phenyl ring to the 1-position of a ribose or deoxyribose sugar moiety. The resulting nucleoside analogue is typically used as a photocrosslinking group when inserted into a polynucleotide as a replacement for one or more of the complementary nucleoside bases present in a probe used in a hybridization assay.
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