Method of aligning, compensating, and calibrating a flow cytometer for analysis of samples, and microbead standards kit therefor
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United States of America Patent
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Mar 3, 1992
Issued Date -
N/A
app pub date -
Jun 30, 1989
filing date -
Dec 24, 1984
priority date (Note) -
Expired
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Abstract
A kit of highly uniform size microbead standards for flow cytometer alignment, compensation, and/or calibration, comprising a blank microbead population and/or an auto-fluorescent microbead population, together with two or more series of calibrated microbead populations labeled with fluorescent dye(s) which (i) prior to fluorescent dye(s) labeling, match the fluorescence spectra and fluorescence intensity of the blank and/or autofluorescent microbead population, and (ii) after fluorescent dye(s) labeling, match the fluorescence spectra and fluorescence intensity of fluorescently labeled samples to be measured on the flow cytometer. Also disclosed is a corresponding method to align, compensate, and/or calibrate a flow cytometer so as to make measurements on samples comparable and independent of the specific instrument and instrument settings.
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