Process and reagent for the improved quantitative colorimetic determination of hydrogen peroxide
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United States of America Patent
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Mar 10, 1992
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N/A
app pub date -
Jan 19, 1988
filing date -
Dec 20, 1984
priority date (Note) -
Expired
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Abstract
The present invention provides a process for the improved colorimetric determination of hydrogen peroxide as formed by a hydrogen peroxide-producing oxidase, by addition of a chromogenic system and measurement of the colored material formed, wherein superoxide dismutase (E.C. 1.15.1.1) is added to the reagent solution. The present invention also provides a reagent for the improved colorimetric determination of hydrogen peroxide, comprising a hydrogen peroxide-producing oxidase, a chromogenic system, a buffer and optionally adjuvant enzymes, wherein it also contains superoxide dismutase.
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