Nucleic acid probe linked to a label having a terminal cysteine
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United States of America Patent
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Apr 28, 1992
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N/A
app pub date -
Feb 2, 1989
filing date -
Jun 1, 1988
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Abstract
A polynucleotide probe with a label bearing a plurality of signalling moieties. The label is attached to the probe by the reaction of an amino and sulfhydryl reactive hetero bifunctional reagent with the probe and label, the reaction resulting in the oxidation of a sulfhydryl group of the label. The label may be attached to the 5' terminus of the probe, or to modified bases of the probe. Probes constructed according to the invention are useful in detecting target sequences in genomic DNA.
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