Target-dependent synthesis of an artificial gene for the synthesis of a replicatable RNA
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United States of America Patent
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May 12, 1992
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N/A
app pub date -
May 26, 1989
filing date -
May 26, 1989
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Expired
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Abstract
This invention pertains to an improved method for detecting a nucleic acid target sequence with a replicatable RNA reporter system. Two polymerase-mediated reactions are used to generate a target-specific gene containing a DNA sequence for a replicatable RNA. Transcription of the target-specific gene yields a replicatable RNA which is amplified by replication. Synthesis of the gene and the replicatable RNA is strictly dependent upon specific interaction with the target sequence. Consequently, the amplified signal (RNA) is target-dependent and background signal is reduced.
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