US Patent No: 5,112,736

Number of patents in Portfolio can not be more than 2000

DNA sequencing using fluorescence background electroblotting membrane

2 Status Updates

Stats

ATTORNEY / AGENT: (SPONSORED)

Importance

Loading Importance Indicators... loading....

Abstract

See full text

A method for the multiplex sequencing on DNA is disclosed which comprises the electroblotting or specific base terminated DNA fragments, which have been resolved by gel electrophoresis, onto the surface of a neutral non-aromatic polymeric microporous membrane exhibiting low background fluorescence which has been surface modified to contain amino groups. Polypropylene membranes are preferably and the introduction of amino groups is accomplished by subjecting the membrane to radio or microwave frequency plasma discharge in the presence of an aminating agent, preferably ammonia. The membrane, containing physically adsorbed DNA fragments on its surface after the electroblotting, is then treated with crosslinking means such as UV radiation or a glutaraldehyde spray to chemically bind the DNA fragments to the membrane through said smino groups contained on the surface thereof. The DNA fragments chemically bound to the membrane are subjected to hybridization probing with a tagged probe specific to the sequence of the DNA fragments. The tagging may be by either fluorophores or radioisotopes. The tagged probes hybridized to said target DNA fragments are detected and read by laser induced fluorescence detection or autoradiograms. The use of aminated low fluorescent background membranes allows the use of fluorescent detection and reading even when the available amount of DNA to be sequenced is small. The DNA bound to the membrances may be reprobed numerous times.

Loading the Abstract Image... loading....

First Claim

See full text

Family

Loading Family data... loading....

Patent Owner(s)

Patent OwnerAddressTotal Patents
BRIGHAM YOUNG UNIVERSITYPROVO, UT177
UNIVERSITY OF UTAHSALT LAKE CITY, UT790

International Classification(s)

  • [Classification Symbol]
  • [Patents Count]

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Caldwell, Karin D Salt Lake City, UT 15 749
Chu, Tun-Jen Salt Lake City, UT 12 494
Pitt, William G Orem, UT 10 201

Cited Art Landscape

Patent Info (Count) # Cites Year
 
SHIN-ETSU CHEMICAL CO., LTD. (1)
* 4,560,458 Method for improving surface properties of a shaped article of a synthetic resin 15 1985
 
CHIRON DIAGNOSTICS CORPORATION (1)
* 4,775,619 Polynucleotide determination with selectable cleavage sites 182 1984
 
PRESIDENT AND FELLOWS OF HARVARD COLLEGE (1)
* 4,942,124 Multiplex sequencing 104 1988
* Cited By Examiner

Patent Citation Ranking

Forward Cite Landscape

Patent Info (Count) # Cites Year
 
BECKMAN COULTER, INC. (2)
6,861,214 Immobilization of biopolymers to aminated substrates by direct adsorption 8 2000
7,494,776 Labeled complementary oligonucleotides to detect oligonucleotide-linked ligands 5 2005
 
AVANTRA BIOSCIENCES CORPORATION (1)
7,384,742 Substrates for isolating reacting and microscopically analyzing materials 1 2003
 
ISIS PHARMACEUTICALS, INC. (7)
* 6,558,633 Chemical reaction apparatus and methods 3 1994
* 6,005,096 Pyrimidine derivatives 142 1995
* 5,925,732 Chemical reaction apparatus for performing multiple reaction on a surface and collecting the product 40 1997
6,277,334 Chemical synthesis apparatus employing a droplet generator 13 1999
6,617,437 Pyrimidine derivatives for labeled binding partners 0 1999
* 2001/0028,866 Chemical synthesis apparatus employing a droplet generator 6 2001
6,967,079 Pyrimidine derivatives for labeled binding partners 0 2002
 
BECKMAN INSTRUMENTS, INC. (3)
* 5,429,807 Method and apparatus for creating biopolymer arrays on a solid support surface 165 1993
* 5,554,501 Biopolymer synthesis using surface activated biaxially oriented polypropylene 38 1993
* 5,583,211 Surface activated organic polymers useful for location - specific attachment of nucleic acids, peptides, proteins and oligosaccharides 40 1994
 
GE HEALTHCARE BIO-SCIENCES AB (1)
7,811,435 Method for scanning gels and gel folder for use in the method 0 2005
 
CALIFORNIA INSTITUTE OF TECHNOLOGY (3)
7,297,518 Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension 96 2002
7,462,449 Methods and apparatuses for analyzing polynucleotide sequences 12 2004
7,981,604 Methods and kits for analyzing polynucleotide sequences 5 2005
 
HITACHI, LTD. (1)
* 5,882,863 Method of measuring nucleic acids and reagent used therefor 16 1996
 
UNIVERSITY OF UTAH (1)
* 5,470,710 Automated hybridization/imaging device for fluorescent multiplex DNA sequencing 110 1993
 
HITACHI ELECTRONICS ENGINEERING CO., LTD. (1)
* 5,552,322 DNA base sequencer 15 1994
 
APPLIED BIOSYSTEMS, LLC (2)
* 6,124,092 Multiplex polynucleotide capture methods and compositions 35 1997
6,514,699 Multiplex polynucleotide capture methods and compositions 11 2000
 
TOYOTA JIDOSHA KABUSHIKI KAISHA (1)
* 7,223,568 Methods for determining nucleotide sequences of single nucleic acid molecules 1 2001
 
BIOLYTIC LAB PERFORMANCE, INC. (1)
9,069,358 System for controlling and optimizing reactions in solid phase synthesis of small molecules 0 2013
 
Genetec Corporation (1)
* 5,451,500 Device for processing biological specimens for analysis of nucleic acids 151 1992
 
SEQUENOM, INC. (3)
6,436,635 Solid phase sequencing of double-stranded nucleic acids 87 1996
7,803,529 Solid phase sequencing of biopolymers 2 1999
8,758,995 Solid phase sequencing of biopolymers 0 2010
 
THE REGENTS OF THE UNIVERSITY OF MICHIGAN (5)
* 6,117,634 Nucleic acid sequencing and mapping 50 1997
6,197,557 Compositions and methods for analysis of nucleic acids 29 1998
6,537,757 Nucleic acid sequencing and mapping 54 2000
6,762,022 Compositions and methods for analysis of nucleic acids 28 2001
7,270,958 Compositions and methods for analysis of nucleic acids 1 2004
 
DE VOS, GERRITT J. (1)
* 6,231,741 Gel cassette and electrophoresis device 6 1997
 
Affymetrix, Inc. (2)
7,329,496 Sequencing of surface immobilized polymers utilizing microflourescence detection 13 2006
7,459,275 Sequencing of surface immobilized polymers utilizing microfluorescence detection 13 2006
 
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY (2)
6,291,170 Multi-genes expression profile 48 1999
7,049,102 Multi-gene expression profile 0 2000
 
MCLUEN DESIGN, INC. (11)
6,270,730 Multi-well rotary synthesizer 13 1998
8,158,085 Multi-well rotary synthesizer 2 2000
7,192,558 Multi-well rotary synthesizer 0 2000
7,150,998 Multi-well rotary synthesizer 0 2000
* 2001/0007,644 Multi-well rotary synthesizer 10 2000
* 2001/0001,035 Multi-well rotary synthesizer 3 2000
* 2001/0000,723 Multi-well rotary synthesizer 3 2000
6,811,755 Multi-well rotary synthesizer 7 2001
8,147,776 Multi-well rotary synthesizer 2 2008
8,404,196 Multi-well rotary synthesizer 0 2012
8,747,780 Multi-well rotary synthesizer 0 2013
 
PROTEINSIMPLE (1)
6,271,042 Biochip detection system 13 1998
 
COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES (1)
* 2012/0009,560 Method For Purifying Nucleic Acids From Microorganisms Present In Liquid Samples 0 2009
 
SCHNEIDER (USA) INC. (1)
* 6,120,904 Medical device coated with interpenetrating network of hydrogel polymers 414 1999
 
Stichting Katholieke Universiteit, The University Medical Centre Nijmegen (3)
7,960,109 mRNA ratios in urinary sediments and/or urine as a prognostic and/or theranostic marker for prostate cancer 6 2005
8,257,924 mRNA ratios in urinary sediments and/or urine as a prognostic and/or theranostic marker for prostate cancer 1 2011
9,096,907 Prostate cancer prognostic compositions and kits 0 2013
 
CLINICAL MICROARRAYS, INC. (1)
7,297,497 Substrates for isolating, reacting and microscopically analyzing materials 0 2006
 
UNIVERSITY HOSPITAL NIJMEGEN (1)
8,551,699 PCA3, PCA3 genes, and methods of use 0 2009
 
DIAGNOCURE INC (7)
* 2005/0282,170 Method to detect prostate cancer in a sample 13 2004
7,655,408 PCA3 messenger RNA species in benign and malignant prostate tissues 0 2008
8,192,931 Method to detect prostate cancer in a sample 1 2009
7,927,806 Distinguishing PCA3 messenger RNA species in benign and malignant prostate tissues 0 2009
8,241,848 Distinguishing PCA3 messenger RNA species in benign and malignant prostate tissues 0 2011
8,546,551 Method to detect prostate cancer in a sample 0 2012
8,618,276 Distinguishing PCA3 messenger RNA species in benign and malignant prostate tissues 0 2012
 
LIFE TECHNOLOGIES CORPORATION (2)
7,645,596 Method of determining the nucleotide sequence of oligonucleotides and DNA molecules 28 2004
9,096,898 Method of determining the nucleotide sequence of oligonucleotides and DNA molecules 0 2010
 
FLUIDIGM CORPORATION (10)
7,169,560 Short cycle methods for sequencing polynucleotides 125 2004
7,220,549 Stabilizing a nucleic acid for nucleic acid sequencing 44 2004
7,482,120 Methods and compositions for improving fidelity in a nucleic acid synthesis reaction 6 2005
7,635,562 Methods and devices for nucleic acid sequence determination 17 2005
7,666,593 Single molecule sequencing of captured nucleic acids 19 2005
7,476,734 Nucleotide analogs 10 2005
7,397,546 Systems and methods for reducing detected intensity non-uniformity in a laser beam 2 2006
7,491,498 Short cycle methods for sequencing polynucleotides 6 2006
7,897,345 Short cycle methods for sequencing polynucleotides 1 2009
9,012,144 Short cycle methods for sequencing polynucleotides 0 2011
 
HITACHI CHEMICAL RESEARCH CENTER, INC. (1)
* 5,545,528 Rapid screening method of gene amplification products in polypropylene plates 36 1995
 
EMD MILLIPORE CORPORATION (2)
8,132,676 Hydrophilic, high protein binding, low fluorescence, western blotting membrane 0 2009
8,143,067 Hydrophilic, high protein binding, low fluorescence, western blotting membrane 1 2010
 
TRUSTEES OF BOSTON UNIVERSITY (5)
* 5,795,714 Method for replicating an array of nucleic acid probes 169 1993
* 5,503,980 Positional sequencing by hybridization 237 1994
* 5,631,134 Methods of preparing probe array by hybridation 111 1995
* 6,007,987 Positional sequencing by hybridization 54 1996
7,319,003 Arrays of probes for positional sequencing by hybridization 1 1998
 
Difco Laboratories Incorporated (1)
* 5,340,747 Diagnostic microbiological testing apparatus and method 124 1993
 
NATIONAL UNIVERSITY CORPORATION TOYOHASHI UNIVERSITY OF TECHNOLOGY (10%) (2)
* 8,778,154 Method of measuring microparticles having nucleic acid and apparatus therefor 0 2009
* 2010/0089,754 METHOD OF MEASURING MICROPARTICLES HAVING NUCLEIC ACID AND APPARATUS THEREFOR 0 2009
 
BOSTON SCIENTIFIC SCIMED, INC. (9)
* 6,017,577 Slippery, tenaciously adhering hydrophilic polyurethane hydrogel coatings, coated polymer substrate materials, and coated medical devices 101 1995
* 5,919,570 Slippery, tenaciously adhering hydrogel coatings containing a polyurethane-urea polymer hydrogel commingled with a poly(N-vinylpyrrolidone) polymer hydrogel, coated polymer and metal substrate materials, and coated medical devices 72 1995
* 5,662,960 Process for producing slippery, tenaciously adhering hydrogel coatings containing a polyurethane-urea polymer hydrogel commingled with a poly (n-vinylpyrrolidone) polymer hydrogel 67 1995
* 5,576,072 Process for producing slippery, tenaciously adhering hydrogel coatings containing a polyurethane-urea polymer hydrogel commingled with at least one other, dissimilar polymer hydrogel 90 1995
* 5,849,368 Process for hydrophilicization of hydrophobic polymers 60 1997
* 6,080,488 Process for preparation of slippery, tenaciously adhering, hydrophilic polyurethane hydrogel coating, coated polymer and metal substrate materials, and coated medical devices 360 1998
* 6,030,656 Process for the preparation of slippery, tenaciously adhering, hydrophilic polyurethane hydrogel coatings, coated metal substrate materials, and coated medical devices 22 1998
* 6,040,058 Slippery, tenaciously adhering hydrophilic polyurethane hydrogel coatings, coated metal substrate materials, and coated medical devices 17 1998
6,265,016 Process for the preparation of slippery, tenaciously adhering, hydrophilic polyurethane hydrogel coatings, coated polymer and metal substrate materials, and coated medical devices 58 2000
 
PHARMACIA CORPORATION (1)
* 2004/0105,883 Pharmaceutical dosage form capable of maintaining stable dissolution profile upon storage 0 2003
* Cited By Examiner