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Abstract

Method and apparatus for use in determining the concentration of a selected analyte in a body-fluid sample. As the fluid sample is applied to a wettable, absorbent reaction pad, the extent of sample wetting of the pad is monitored by surface reflectance. This surface monitoring is used to control the volume of sample applied to the pad, to prevent overfilling the pad and to allow determination of the final sample volume applied. From the known sample volume and amount of analyte, the concentration of analyte in the sample can be accurately calculated in reaction components, trapping agents, matrix configuration, and analyte tested can be made.

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First Claim