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Abstract

A microbial shuttle vector is disclosed which is independently replicative in bacterial cells and mammalian cells and includes in its DNA sequence bacterial plasmid sequences allowing selection and replication in bacterial cells, an SV40 viral origin of replication, and either an SV40 functional 'early gene' promoter and functional 'early gene' terminator or an SV40 functional 'late gene' promoter and functional 'late gene' terminator, the vector having a unique restriction endonuclease enzyme recognition site between the promoter and terminator for insertion of an exogenous gene. The presence of restriction endonuclease enzyme recognition sites facilitative of insertion of a viral functional 'late gene' into the 'early gene' promoter/terminator vector in a single step allows for conversion of the shuttle vector into a lytic vector of an exogenous gene. The presence of restriction endonuclease enzyme recognition sites facilitative of insertion of a viral functional 'late gene' into the 'late gene' promoter/terminator vector in a single step allows for conversion of the shuttle vector into a lytic vector.

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First Claim