US Patent No: 5,118,801

Number of patents in Portfolio can not be more than 2000

Nucleic acid process containing improved molecular switch

Stats

ATTORNEY / AGENT: (SPONSORED)

Importance

Loading Importance Indicators... loading....

Abstract

See full text

A probe for the detection of a nucleic acid target sequence containing a molecular switch comprising three essential elements: a probe sequence of 20-60 nucleotides surrounded by switch sequences of 10-40 nucleotides which are complementary to each other, wherein the state of the switch is useful for selectively generating a detectable signal if the probe is hybridized to a target; also, assays and kits utilizing such probes.

Loading the Abstract Image... loading....

First Claim

See full text

all claims..

Related Publications

Loading Related Publications... loading....

Patent Owner(s)

Patent OwnerAddressTotal Patents
THE SALK INSTITUTE FOR BIOLOGICAL STUDIESLA JOLLA, CA485
UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEYNEW BRUNSWICK, NJ156
PUBLIC HEALTH RESEARCH INSTITUTENEW YORK, NY4

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Buyoli, Hilda M L Col Marte, MX 2 293
Guerra, Cesar E Guilford, CT 8 362
Kramer, Fred R Riverdale, NY 30 1344
Lizardi, Paul M Cuernavaca, MX 52 2399
Tyagi, Sanjay New York, NY 20 1175

Cited Art Landscape

Patent Info (Count) # Cites Year
 
Eli Lilly and Company (1)
4,710,464 Transcription terminators 10 1984
 
Genentech, Inc. (1)
4,551,433 Microbial hybrid promoters 131 1982
 
GENETICS INSTITUTE, LLC (1)
4,725,537 Assay, reagent and kit employing nucleic acid strand displacement and restriction endonuclease cleavage 41 1985
 
ROCHE MOLECULAR SYSTEMS, INC. (1)
4,683,202 Process for amplifying nucleic acid sequences 3106 1985

Patent Citation Ranking

Forward Cite Landscape

Patent Info (Count) # Cites Year
 
GEN-PROBE INCORPORATED (28)
5,424,413 Branched nucleic acid probes 92 1992
5,451,503 Method for use of branched nucleic acid probes 42 1994
6,025,133 Promoter-sequestered oligonucleoside and method of use 18 1996
6,361,945 Molecular torches 65 1999
7,070,925 Method for determining the presence of an RNA analyte in a sample using a modified oligonucleotide probe 4 2000
7,399,852 Kits and reaction mixtures containing modified probe molecules 6 2001
6,534,274 Molecular torches 35 2001
6,849,412 Molecular torches 19 2003
6,835,542 Molecular torches 13 2003
7,582,470 Device for amplifying and detecting a target nucleic acid 0 2003
7,252,947 Molecular torches and their use under denaturing conditions 0 2005
7,250,259 Molecular torches and their use under strand displacement conditions 0 2005
8,034,554 Methods and compositions to detect nucleic acids in a biological sample 0 2005
7,510,837 Sample preparation method incorporating an alkaline shock 2 2006
7,588,897 nucleic acid 0 2007
7,563,579 Method for detecting a structured target 0 2007
7,495,093 Amplification and detection method 0 2007
7,572,588 Modified probe molecules having self-complementary regions 0 2007
8,202,978 Compositions for detecting group A streptococci 0 2008
7,595,164 nucleic acid 0 2008
8,097,409 Kits for detecting group B Streptococci 0 2009
8,703,421 0 2009
7,670,780 nucleic acid 2 2009
8,399,222 Compositions and methods for detecting small RNAs, and uses thereof 0 2009
8,071,301 Method of isolating nucleic acids from a biological sample 1 2010
8,551,766 Methods and compositions to detect nucleic acids in a biological sample 0 2010
8,460,869 Methods and compositions to detect nucleic acids in a biological sample 0 2011
8,420,317 Alkaline shock-based method of processing a biological sample 0 2011
 
ROCHE DIAGNOSTICS OPERATIONS, INC. (12)
7,541,448 Oligonucleotides containing molecular rods 1 2004
7,687,260 Imaging fluorescence signals using telecentric optics 0 2006
7,369,227 Imaging fluorescence signals using telecentricity 0 2006
7,759,469 Labeling reagent 0 2006
7,569,367 Nucleic acid preparation from whole blood for use in diagnosis of transmissible spongiform encephalopathy 0 2006
7,781,165 Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification 3 2006
8,119,353 Rapid one-step reverse transcriptase PCR 0 2008
7,741,472 Polynucleotide containing a phosphate mimetic 0 2008
8,026,058 PCR hot start by magnesium sequestration 0 2008
7,910,720 Polyanion for improved nucleic acid amplification 3 2009
8,652,811 Dry composition of reaction compounds with stabilized polymerase 0 2009
8,357,490 Integrated instrument performing synthesis and amplification, and a system and method thereof 0 2010
 
Enzo Life Sciences, Inc. (10)
7,241,897 Process for preparing cyanine dye labeling reagents 1 2004
8,357,793 Label target and labeling reagents comprising backbones with at least two consecutive peptide bonds 0 2004
7,863,431 Label target and labeling reagents comprising rigid group backbones 1 2004
7,537,751 Labeling reagents and labeled targets comprising nonmetallic porphyrins 0 2004
7,256,291 Labeling reagents comprising aphenylic analogs of rhodamine dyes 1 2004
7,553,959 Fluorescent dye composition and target labeled therewith 1 2004
7,163,796 Process for detecting the presence or quantity of enzymatic activity in a sample 0 2004
6,949,659 Dye labeling composition 0 2004
7,858,302 Processes for incorporating nucleic acid sequences into an analyte or library of analytes 0 2005
8,247,179 Processes for quantitative or qualitative detection of single-stranded nucleic acids 0 2009
 
BOSTON PROBES, INC. (9)
6,485,901 Methods, kits and compositions pertaining to linear beacons 36 1998
6,355,421 Methods, kits and compositions pertaining to PNA molecular beacons 56 1998
6,361,942 Method, kits and compositions pertaining to detection complexes 14 1999
6,607,889 Methods, kits and compositions pertaining to detection complexes 6 2001
6,528,267 Methods, kits and compositions pertaining to PNA molecular beacons 10 2001
6,649,349 In-situ methods for analyzing target sequences using linear beacons 3 2001
6,949,343 Methods, kits and compositions pertaining to PNA Molecular Beacons 1 2003
7,816,501 Compositions comprising a linked acceptor moiety 0 2003
7,892,741 Methods, kits and compositions pertaining to linear beacons 0 2008
 
SAMSUNG ELECTRONICS CO., LTD. (8)
6,936,496 Nanowire filament 3 2002
7,223,611 Fabrication of nanowires 1 2003
7,132,298 Fabrication of nano-object array 1 2003
7,407,738 Fabrication and use of superlattice 3 2004
7,247,531 Field-effect-transistor multiplexing/demultiplexing architectures and methods of forming the same 0 2004
7,375,012 Method of forming multilayer film 0 2005
7,633,098 Field-effect-transistor multiplexing/demultiplexing architectures 0 2007
7,847,368 Multilayer film with stack of nanometer-scale thicknesses 0 2008
 
ROCHE MOLECULAR SYSTEMS, INC. (7)
7,718,361 Quantitative test for bacterial pathogens 1 2003
8,137,616 System for multi color real time PCR 1 2004
7,920,971 Quantification schemes for quantifying nucleic acids 0 2004
7,981,606 Control for nucleic acid testing 0 2006
8,623,602 Lysis and reverse transcription for MRNA quantification 0 2011
8,592,184 Nucleic acid amplification in the presence of modified randomers 0 2012
8,822,158 Miniaturized, high-throughput nucleic acid analysis 0 2013
 
BIOTIUM, INC. (6)
7,776,567 Dimeric and trimeric nucleic acid dyes, and associated systems and methods 2 2006
7,601,498 Methods of using dyes in association with nucleic acid staining or detection and associated technology 2 2006
7,803,943 Methods of using dyes in association with nucleic acid staining or detection and associated technology 1 2009
8,530,195 Dyes and labeled molecules 0 2010
8,232,050 Methods of using dyes in association with nucleic acid staining or detection and associated technology 2 2010
8,753,814 Methods of using dyes in association with nucleic acid staining or detection and associated technology 0 2012
 
DIAGNOCURE INC. (6)
7,655,408 PCA3 messenger RNA species in benign and malignant prostate tissues 0 2008
8,192,931 Method to detect prostate cancer in a sample 0 2009
7,927,806 Distinguishing PCA3 messenger RNA species in benign and malignant prostate tissues 0 2009
8,241,848 Distinguishing PCA3 messenger RNA species in benign and malignant prostate tissues 0 2011
8,546,551 Method to detect prostate cancer in a sample 0 2012
8,618,276 Distinguishing PCA3 messenger RNA species in benign and malignant prostate tissues 0 2012
 
MOUNT SINAI SCHOOL OF MEDICINE (6)
5,942,391 Nucleic acid amplification method: ramification-extension amplification method (RAM) 102 1996
5,876,924 Nucleic acid amplification method hybridization signal amplification method (HSAM) 108 1996
6,569,647 Nucleic acid amplification method: ramification-extension amplification method (RAM) 32 1999
6,593,086 Nucleic acid amplification methods 4 2000
RE38442 Nucleic acid amplification method hybridization signal amplification method (HSAM) 5 2001
6,855,523 Nucleic acid amplification method: ramification-extension amplification method (RAM) 8 2002
 
ENZO LIFE SCIENCES, INC. C/O ENZO BIOCHEM, INC. (5)
7,166,478 Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses 11 2002
7,323,571 Heterodimeric dye composition 0 2004
7,256,299 Chemiluminescent reagents 1 2004
7,569,695 Dyes for the detection or quantification of desirable target molecules 2 2005
7,737,281 Purine based fluorescent dyes 1 2005
 
HOLOGIC, INC. (5)
6,350,580 Methods for detection of a target nucleic acid using a probe comprising secondary structure 44 2000
6,589,743 Methods for detection of a target nucleic acid using a probe comprising secondary structure 30 2001
7,838,225 Methods for detection of a target nucleic acid by forming a cleavage structure using a reverse transcriptase 0 2006
7,824,859 Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase 1 2006
7,846,661 Methods of detecting an amplified nucleic acid 0 2006
 
MOLECULAR MACHINES, INC. (5)
5,739,305 Nucleotide-directed assembly of bimolecular and multimolecular drugs and devices 7 1995
5,656,739 Nucleotide-directed assembly of bimolecular and multimolecular drugs and devices 14 1995
5,756,296 Nucleotide-directed assembly of bimolecular and multimolecular drugs and devices 6 1995
6,287,765 Methods for detecting and identifying single molecules 195 1998
6,762,025 Single-molecule selection methods and compositions therefrom 28 2001
 
UNIVERSITY OF UTAH (5)
6,303,305 Method for quantification of an analyte 50 1999
6,503,720 Method for quantification of an analyte 16 2001
7,630,837 Real-time gene quantification with internal standards 1 2002
7,153,654 Assay procedure using fluorogenic tracers 1 2002
8,557,532 Diagnosis and treatment of drug-resistant Ewing'S sarcoma 0 2010
 
ANADYS PHARMACEUTICALS, INC. (4)
6,337,183 Screen for compounds with affinity for nucleic acids 1 1996
6,331,392 Screen employing fluorescence anisotropy to identify compounds with affinity for nucleic acids 6 1998
6,569,628 Screen employing fluorescence anisotropy to identify compounds with affinity for nucleic acids 1 2001
6,503,721 Screen for compounds with affinity for nucleic acids 3 2001
 
CALIFORNIA INSTITUTE OF TECHNOLOGY (4)
7,538,202 Enzyme-free isothermal exponential amplification of nucleic acids and nucleic acid analog signals 1 2004
8,497,364 Triggered RNAi 0 2009
8,658,780 Triggered covalent probes for imaging and silencing genetic expression 0 2011
8,507,204 Hybridization chain reaction amplification for in situ imaging 0 2012
 
GILEAD SCIENCES, INC. (4)
5,989,823 Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction 68 1998
6,177,555 Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction 27 1999
6,261,783 Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction 30 2000
6,531,286 Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction 6 2001
 
PHRI PROPERTIES, INC. (4)
5,925,517 Detectably labeled dual conformation oligonucleotide probes, assays and kits 319 1995
6,150,097 Nucleic acid detection probes having non-FRET fluorescence quenching and kits and assays including such probes 156 1997
6,037,130 Wavelength-shifting probes and primers and their use in assays and kits 103 1998
6,103,476 Detectably labeled, dual conformation oligonucleotide probes, assays and kits 103 1999
 
THIEN, DOUGLAS Y. (4)
6,177,243 Nucleic acid sequence detection employing amplification probes 3 1997
6,277,570 Nucleic acid sequence detection employing probes comprising non-nucleosidic coumarin derivatives as polynucleotide-crosslinking agents 12 1998
6,495,676 Nucleic acid sequence detection employing probes comprising non-nucleosidic coumarin derivatives as polynucleotide-crosslinking agents 1 1999
6,590,091 Nucleic acid sequence detection employing amplification probes 14 2001
 
LIFE TECHNOLOGIES CORPORATION (3)
7,537,886 Primers and methods for the detection and discrimination of nucleic acids 21 2000
8,828,700 SSB-polymerase fusion proteins 0 2008
8,394,609 Primers and methods for the detection and discrimination of nucleic acids 0 2009
 
ONCOIMMUNIN, INC. (3)
6,936,687 Compositions for the detection of enzyme activity in biological samples and methods of use thereof 5 1999
7,541,143 Homo-doubly labeled compositions for the detection of enzyme activity in biological samples 1 2004
7,879,574 Compositions for the detection of enzyme activity in biological samples and methods of use thereof 0 2007
 
THIRD WAVE TECHNOLOGIES, INC. (3)
7,851,150 Detection of small nucleic acids 4 2003
8,206,904 Detection of nucleic acids 1 2007
8,609,336 Detection of small nucleic acids 0 2012
 
BRANDEIS UNIVERSITY (2)
6,680,377 Nucleic acid-based detection 34 2000
8,071,734 Nucleic acid-based detection 1 2006
 
CITY OF HOPE (2)
6,200,782 Construction of nucleoprotein based assemblies comprising addressable components for nanoscale assembly and nanoprocessors 2 1997
6,383,784 Construction of nucleoprotein based assemblies comprising addressable components for nanoscale assembly and nanoprocessors 8 1999
 
EMD MILLIPORE CORPORATION (2)
6,117,635 Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon 84 1997
6,090,552 Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon 30 1997
 
HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P. (2)
7,683,435 Misalignment-tolerant multiplexing/demultiplexing architectures 0 2004
7,294,899 Nanowire Filament 0 2005
 
ONCOLMMUNIN, INC. (2)
6,893,868 Homo-doubly labeled compositions for the detection of enzyme activity in biological samples 4 2000
7,312,302 Compositions for the detection of enzyme activity in biological samples and methods of use thereof 2 2001
 
ORTHO-CLINICAL DIAGNOSTICS, INC. (2)
7,262,006 Rapid and efficient capture of DNA from sample without using cell lysing reagent 4 2000
7,615,346 Rapid and efficient capture of DNA from sample without using cell lysing reagent 0 2006
 
RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY (2)
6,277,607 High specificity primers, amplification methods and kits 37 1999
6,365,729 High specificity primers, amplification methods and kits 36 2001
 
STICHTING KATHOLIEKE UNIVERSITEIT, THE UNIVERSITY MEDICAL CENTRE NIJMEGEN (2)
7,960,109 mRNA ratios in urinary sediments and/or urine as a prognostic and/or theranostic marker for prostate cancer 2 2005
8,257,924 mRNA ratios in urinary sediments and/or urine as a prognostic and/or theranostic marker for prostate cancer 0 2011
 
WALLAC OY (2)
7,776,530 Integrated nucleic acid analysis 2 2005
8,252,536 Integrated nucleic acid analysis 0 2010
 
ABBOTT LABORATORIES (1)
5,814,492 Probe masking method of reducing background in an amplification reaction 16 1997
 
ALLELOGIC BIOSCIENCES CORPORATION (1)
7,667,024 Oligonucleotides labeled with a plurality of fluorophores 1 2004
 
BARCLAY, DOUGLAS C. (1)
5,866,336 Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon 205 1997
 
BOSTON PROBEE, INC. (1)
7,422,852 Detection of nucleic acid using linear beacons 1 2003
 
DIASORIN S.P.A. (1)
6,887,662 Oligonucleotides and assemblies thereof useful in the detection of the presence or absence of target nucleic acid sequences in a sample 3 2000
 
EAGLE EYE RESEARCH, INC. (1)
8,043,810 Analyte detection using autocatalytic chain reactions 0 2007
 
PROMEGA CORPORATION (1)
6,100,024 Methods and compositions for nucleic acid detection by target extension and probe amplification 9 1991
 
ROCHE DIAGNOSTICS GMBH (1)
7,122,355 Composition and method for hot start nucleic acid amplification 0 2002
 
SPARTAN BIOSCIENCE INC. (1)
7,466,908 System for rapid nucleic acid amplification and detection 0 2005
 
UNIVERSITY HOSPITAL NIJMEGEN (1)
8,551,699 PCA3, PCA3 genes, and methods of use 0 2009
 
UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEY (1)
6,043,028 Method for synthesizing single-stranded stem-loop DNA's the products and uses therefore 0 1995
 
ZENECA LIMITED (1)
6,326,145 Methods for detecting target nucleic acid sequences 35 1998
 
Other [Check patent profile for assignment information] (1)
8,632,999 Nucleic acid amplification methods 0 2003