Amperometric method for the quantitative determination of 1,4-dihydronicotinamide adenine dinucleotide (NADH) in solution
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United States of America Patent
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Jun 16, 1992
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N/A
app pub date -
Feb 24, 1989
filing date -
May 1, 1987
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Expired
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Abstract
A method is disclosed for the quantitative determination of 1,4-dihydronicotinamide adenisne dinucleotide (NADH) in solution. The method comprises contacting the NADH-containing solution with an activated carbon electrode, maintaining the carbon electrode at a controlled, fixed potential effective to cause oxidation of NADH at the electrode surface, and measuring the current output from the carbon electrode, wherein there is used a noble metal containing preferably a platinized or palladized activated carbon electrode comprising a porous, heterogeneous, resin-bonded layer of activated carbon or graphite particles comprising the finely divided noble metal preadsorbed thereon and bonded together with a natural or synthetic resin binder, preferably a hydrophobic resin such as polytetrafluoroethylene.
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