Process for detecting polymorphisms on the basis of nucleotide differences
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United States of America Patent
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Jun 30, 1992
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N/A
app pub date -
Mar 14, 1990
filing date -
Mar 14, 1990
priority date (Note) -
Expired
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Abstract
A process is provided for detecting polymorphisms on the basis of nucleotide differences in random segments of the nucleic acid by performing a primer extension reaction on the nucleic acids and comparing the extension reaction products. The random nucleic acid segment can be amplified by first dissociating the extension product from the template and contacting the dissociated extension product with a primer under conditions such that an amplification extension product is synthesized using the dissociated extension product as a template. Differences in the extension product are useful as markers in constructing genetic maps and as markers to distinguish or identify individuals.
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