Method for determining the .beta.-subunit of human prolyl hydroxylase by radioimmunoassay to detect hepatic disease
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United States of America Patent
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Jul 14, 1992
Grant Date -
N/A
app pub date -
Dec 28, 1989
filing date -
Aug 16, 1985
priority date (Note) -
Expired
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Abstract
A method for determining human prolyl hydroxylase by radioimmunoassay according to the sandwich method wherein a monoclonal antibody to human prolyl hydroxylase and polyclonal antibody to human prolyl hydroxylase are used, characterized in that a monoclonal antibody to human prolyl hydroxylase is used as at least one of the antibodies which are to be coated on a solid support and to be labeled with a radioactive element. This method is simple and operable with small amounts of samples and gives exact results. Thus, this method is useful for the diagnosis of hepatic diseases. A monoclonal antibody specific to the .beta.-subunit of human prolyl hydroxylase is used to test for the human prolyl hydroxylase.
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