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Abstract

In a method for measuring protein content of whole grain kernels with a near infrared spectrophotometer, reflectance measurements are made at wavelengths shorter than 1600 nanometers. The wavelengths are selected to correspond with absorbance bands of the constituents of the sample. The measurements are encoded into absorbance data and are normalized by subtracting the absorbance value at a first wavelength and then dividing the resulting data by the value at a second wavelength. In accordance with one embodiment, the normalized data is substituted in a formula having coefficients determined by regression from samples having known percentages of protein in the sample to yield the percentage of protein in the unknown sample. In accordance with a second embodiment, the second differential of the absorbance data is determined and the resulting differential values are inserted in the formula having coefficients obtained from the known samples to yield the percentage or protein in the unknown sample.

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First Claim