Method to purify basic fibroblast growth factor
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United States of America Patent
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Aug 4, 1992
Grant Date -
N/A
app pub date -
Apr 4, 1990
filing date -
Apr 4, 1990
priority date (Note) -
Expired
status (Latency Note)
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Abstract
This invention relates to a method of isolating and purifying proteins such as fibroblast growth factor using metal chelate affinity column chromatography and without the use of heparin. Also disclosed is a method of recovering bFGF multimers and purifying bFGF using metal chelate affinity column chromatography in the absence of heparin.
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