Use of the GAL1 yeast promoter
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United States of America Patent
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Aug 18, 1992
Issued Date -
N/A
app pub date -
Dec 13, 1988
filing date -
Feb 28, 1983
priority date (Note) -
Expired
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Abstract
A DNA segment contains a GAL1 promoter of Saccharomyces cerevisae linked to a gene other than the galoctokinase gene, for directing the expression of the gene within a yeast cell. A GAL1 promoter portion of Saccharomyces cerevisae is linked to a foreign DNA segment for use in expressing a desired protein. Yeast cells containing the GAL1 promoter linked to a foreign DNA segment are grown in a medium containing glucose, wherein the yeast cells metabolize the glucose and are permitted to express a polypeptide when galactose is present in the medium.
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