Method of producing thrombin from factor II using calcium ions for the conversion on an anion exchanger
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United States of America Patent
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Sep 1, 1992
Grant Date -
N/A
app pub date -
Dec 20, 1989
filing date -
Dec 22, 1988
priority date (Note) -
Expired
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Abstract
A method of producing thrombin from Factor II (prothrombin) comprising the steps of: a) applying a solution of citrated plasma or citrated plasma fraction containing Factor II onto an equilibrated anion exchanger to bind Factor II thereto; b) applying a solution containing calcium ions to the exchanger to convert the Factor II to thrombin, and c) selectively eluting the thrombin from the carrier.
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