Antibodies to ligand analogues and their utility in ligand-receptor assays
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United States of America Patent
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Sep 1, 1992
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N/A
app pub date -
Sep 14, 1990
filing date -
Sep 14, 1990
priority date (Note) -
Expired
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Abstract
Means for the detection of free ligand analogue conjugate in fluids from competitive ligand-receptor assay processes. Ligand analogue antibodies that bind the ligand analogue conjugate with substantially greater affinity than their affinity for target ligand are selected and used in competitive ligand-receptor assay processes to bind the free fraction of the ligand analogue conjugate. This means permits the detection of the free fraction of ligand analogue conjugate even in the presence of substantially higher concentrations of free target ligand. For the purposes of the present invention, ligand analogue antibodies are antibodies that exhibit at least 100.times.greater affinity for the ligand analogue conjugate compared to their affinity for the target ligand.
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