Process for synthesizing oligonucleotides and their analogs adaptable to large scale syntheses
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United States of America Patent
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Sep 22, 1992
Grant Date -
N/A
app pub date -
Apr 6, 1989
filing date -
Apr 6, 1989
priority date (Note) -
Expired
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Abstract
A process of producing synthetic oligonucleotides on a small or large scale using H-phosphonate nucleoside monomers is described. The process can be used to synthesize oligonucleotides of any length, including oligodeoxyribonucleotides and oligoribonucleotides. The process results in a coupling efficiency of greater than 97% and consumes only two to three equivalents of monomer to activator per coupling reaction. In addition, the process does not require a separate capping step and capping reagent because the activating reagent serves a self-capping function thereby preventing elongation of failed sequences. The H-phosphonate linkages of the fully synthesized oligonucleotide can be oxidized with a variety of reagents to obtain either phosphate diester or other types of modified oligonucleotides.
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