Assay device and method using a signal-modulating compound
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United States of America Patent
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Oct 20, 1992
Grant Date -
N/A
app pub date -
Mar 8, 1989
filing date -
Mar 8, 1989
priority date (Note) -
Expired
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Abstract
An assay device and method for determining the concentration of an analyte. Analyte introduced by liquid absorption into the matrix is used to generate H2O2, via an analyte-specific oxidase, and the H.sub.2 O.sub.2 is utilized by peroxidase in the presence of peroxidase to convert a substrate reagent to a colored reaction product. A trapping compound in the matrix competes with the substrate reagent, in the presence of the peroxidase enzyme, to produce a silent reaction product, thus proportionately reducing the amount of signal reaction product generated by a given amount of analyte. Detection of a wider range of analyte concentrations is therefore possible.
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