Process for preparing tissue for transplantation
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United States of America Patent
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Nov 3, 1992
Grant Date -
N/A
app pub date -
May 14, 1991
filing date -
May 14, 1991
priority date (Note) -
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Abstract
A method is disclosed for preparing a transplantable tissue which has been cryopreserved with an intracellular cryoprotectant and then thawed. After thawing, the tissue is treated with a diluent (or eluent) solution to reduce the level of cryoprotectant in the cells to a substantially non-toxic level. This is conducted using a single dilution step. Cryoprotectants which are removable in this manner include dimethylsulfoxide, glycerol, propanediol and other compounds which penetrate the cells. The dilution step does not require end point titration and can be conducted over a time period greater than about 5 minutes.
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