Analytical assay method
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United States of America Patent
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Nov 24, 1992
Grant Date -
N/A
app pub date -
Jul 19, 1989
filing date -
Jul 19, 1989
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A method for determining the amount of an analyte in a sample fluid utilizes a multilayer assay element which comprises at least one reagent layer and a light-blocking layer. The assay method includes the steps of optically reading a signal producing species, e.g. a fluorescent label, a first time before the sample fluid is applied to the assay element and a second time, at the same wavelength and in the same location within the assay element, after the sample fluid has been applied to the assay element and the sample analyte has interacted with the reagent(s) present in the assay element. The ratio of the two signals is taken and compared with that for known amounts of the analyte to determine the amount of analyte in the sample fluid.
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