Method and apparatus for optically detecting presence of immunological components
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United States of America Patent
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Dec 8, 1992
Grant Date -
N/A
app pub date -
Aug 30, 1990
filing date -
Aug 30, 1990
priority date (Note) -
Expired
status (Latency Note)
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Abstract
An optical immunosensing device for detecting the presence of an analyte in a liquid sample is fabricated by coating iron phosphate onto a silicon substrate and then etching the iron phosphate layer to obtain steps of varying thickness. Then, a first mip, capable of immunological reaction with the analyte, is immobilized on the iron phosphate steps. In the preferred embodiment, the first mip is chemically bound to the iron phosphate step surface. The presence of the analyte in the liquid sample is detected by visually observing color changes in the steps after contact of the detection zone with the liquid sample. A change in step color is related to increased thickness which can only result from the binding of the analyte to the first mip immobilized on the iron phosphate step. Aluminum phosphate can also be added to the iron phosphate.
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