Methods of measuring membrane potential using J-aggregate forming dyes
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United States of America Patent
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Dec 8, 1992
Grant Date -
N/A
app pub date -
Jun 9, 1989
filing date -
Jun 9, 1989
priority date (Note) -
Expired
status (Latency Note)
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Abstract
Disclosed is a method of detecting a localized biochemical event in a cell, wherein said event results in a change in the membrane potential of that cell. The method includes treating the cell with a composition containing a lipophilic, cationic dye having a delocalized positive charge and the ability to undergo multiple changes in fluorescence spectra upon aggregation. The cell is treated with the dye for a time sufficient to enable the dye to associate with the cell. Dye which has not associated with the cell is then removed. Fluorescence is observed when the cell is exposed to light having a wavelength suitable for exciting the dye. The spectrum obtained is indicative of the relative membrane potential of said cell, and a change in that membrane potential being indicative of the occurrence of a biochemical event.
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