Method of detecting HIV protease activity
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United States of America Patent
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Dec 15, 1992
Issued Date -
N/A
app pub date -
Apr 4, 1991
filing date -
Sep 13, 1990
priority date (Note) -
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Abstract
A method for identifying compounds that inhibit HIV protease is disclosed. A substrate that comprises an HIV protease cleavage site is combined with HIV protease and test compounds. Cleavage of the substrate indicates protease activity and can be detected using antibodies against a cleavage product which do not cross react with uncleaved substrate. A method of detecting the presence of anti-HIV protease antibodies in a sample is also disclosed. A substrate is combined with the sample and HIV protease. Detection of substrate cleavage indicates that the protease is active and that there is an absence of neutralizing anti-HIV protease antibodies.
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