Method for maintaining immobilized glucose isomerase activity during continuous isomerization of glucose to fructose
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United States of America Patent
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Jan 5, 1993
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Sep 16, 1991
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Aug 2, 1984
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Abstract
This invention provides a method for the maintenance of continuous activity of an immobilized enzyme reaction by initially underloading an adsorbent with a desired enzyme, monitoring the activity of the enzyme and periodically adding fresh enzyme to maintain a constant, continuous level of activity until the maximum carrying capacity of the support is reached. The method is preferably carried out when continuously isomerizing glucose to fructose with glucose isomerase adsorbed to a weakly basic anion exchange resin.
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